Exploiting Emotions via Composite Pretrained Embedding and Ensemble Language Model

Decisions in the modern era are based on more than just the available data; they also incorporate feedback from online sources. Processing reviews known as Sentiment analysis (SA) or Emotion analysis. Understanding the user's perspective and routines is crucial now-a-days for multiple reasons. It is used by both businesses and governments to make strategic decisions. Various architectural and vector embedding strategies have been developed for SA processing. Accurate representation of text is crucial for automatic SA. Due to the large number of languages spoken and written,  polysemy and syntactic or semantic issues were common. To get around these problems, we developed effective composite embedding (ECE), a method that combines the advantages of vector embedding techniques that are either context-independent (like glove & fasttext) or context-aware (like  XLNet) to effectively represent the features needed for processing.  To improve the performace towards emotion or  sentiment we proposed stacked ensemble model of deep lanugae models.ECE with Ensembled model is evaluated on balanced  dataset to prove that it is a reliable embedding technique and a generalised model for SA.In order to evaluate ECE, cutting-edge ML and Deep net language models are deployed and comapared. The model is evaluated using benchmark datset such as  MR, Kindle along with realtime tweet dataset of user complaints . LIME is used to verify the model's predictions and to provide statistical results for sentence.The model with ECE embedding provides state-of-art results with real time dataset as well

The Antioxidant Activity of the Leaves of Barleria grandiflora Dalz. (Acanthaceae)

Aqueous and hydro alcoholic extracts of the leaves of Barleria grandiflora Dalz. were evaluated for the antioxidant activity by the FTC and TBA methods. The results obtained in the present study indicate that the leaves of Barleria grandiflora are potential source of natural antioxidants. Initial phytochemical screenings of the extracts have shown the presence of flavanoids, tannins, saponins, carbohydrates and aminoacid

N 1 -methylpseudouridylation of mRNA causes +1 ribosomal frameshifting

In vitro-transcribed (IVT) mRNAs are modalities that can combat human disease, exemplified by their use as vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). IVT mRNAs are transfected into target cells, where they are translated into recombinant protein, and the biological activity or immunogenicity of the encoded protein exerts an intended therapeutic effect1, 2. Modified ribonucleotides are commonly incorporated into therapeutic IVT mRNAs to decrease their innate immunogenicity3–5, but their effects on mRNA translation fidelity have not been fully explored. Here we demonstrate that incorporation of N1-methylpseudouridine into mRNA results in +1 ribosomal frameshifting in vitro and that cellular immunity in mice and humans to +1 frameshifted products from BNT162b2 vaccine mRNA translation occurs after vaccination. The +1 ribosome frameshifting observed is probably a consequence of N1-methylpseudouridine-induced ribosome stalling during IVT mRNA translation, with frameshifting occurring at ribosome slippery sequences. However, we demonstrate that synonymous targeting of such slippery sequences provides an effective strategy to reduce the production of frameshifted products. Overall, these data increase our understanding of how modified ribonucleotides affect the fidelity of mRNA translation, and although there are no adverse outcomes reported from mistranslation of mRNA-based SARS-CoV-2 vaccines in humans, these data highlight potential off-target effects for future mRNA-based therapeutics and demonstrate the requirement for sequence optimization

Uncovering Enhancer Functions Using the α-Globin Locus

Over the last three decades, studies of the α- and β-globin genes clusters have led to elucidation of the general principles of mammalian gene regulation, such as RNA stability, termination of transcription, and, more importantly, the identification of remote regulatory elements. More recently, detailed studies of α-globin regulation, using both mouse and human loci, allowed the dissection of the sequential order in which transcription factors are recruited to the locus during lineage specification. These studies demonstrated the importance of the remote regulatory elements in the recruitment of RNA polymerase II (PolII) together with their role in the generation of intrachromosomal loops within the locus and the removal of polycomb complexes during differentiation. The multiple roles attributed to remote regulatory elements that have emerged from these studies will be discussed

PGS analysis of over 100,000 blastocysts using high resolution next generation sequencing

To determine the types and frequency of abnormalities in human blastocyst detected by high resolution next generation sequencing (hr-NGS) stratified by age and laboratory

Current research trends on emerging contaminants pharmaceutical and personal care products (PPCPs): A comprehensive review

Pharmaceutical and personnel care products (PPCPs) from wastewater are a potential hazard to the human health and wildlife, and their occurrence in wastewater has caught the concern of researchers recently. To deal with PPCPs, various treatment technologies have been evolved such as physical, biological, and chemical methods. Nevertheless, modern and efficient techniques such as advance oxidation processes (AOPs) demand expensive chemicals and energy, which ultimately leads to a high treatment cost. Therefore, integration of chemical techniques with biological processes has been recently suggested to decrease the expenses. Furthermore, combining ozonation with activated carbon (AC) can significantly enhance the removal efficiency. There are some other emerging technologies of lower operational cost like photo-Fenton method and solar radiation-based methods as well as constructed wetland, which are promising. However, feasibility and practicality in pilot-scale have not been estimated for most of these advanced treatment technologies. In this context, the present review work explores the treatment of emerging PPCPs in wastewater, via available conventional, non-conventional, and integrated technologies. Furthermore, this work focused on the state-of-art technologies via an extensive literature search, highlights the limitations and challenges of the prevailing commercial technologies. Finally, this work provides a brief discussion and offers future research directions on technologies needed for treatment of wastewater containing PPCPs, accompanied by techno-economic feasibility assessment

Thermally stimulated discharge conductivity of PVC thin film

375-382The conductivity and thermally stimulated discharge currents (TSODC) in polyvinyl chloride (PVC) are studied in virgin and electret thin film samples. The V-I characteristics of the films at different temperatures reveal that there are three distinct regions, namely ohmic, square law and power regions. In the square law region, space charge limited conduction is observed. The possibility of Poole-Frenkel or Richardson-Schottky mechanism is also studied. The field-lowering coefficient (β) is calculated. The Richardson-Schottky mechanism is observed. From - log σ vs. 1/T plot, the activation energy is estimated. The electrical conductivity is found to increase with decreasing thickness. TSDC and conductivity are measured in the film of electrets in 313-333 K. Single relaxation peak is observed in the discharge current vs. Temperature plot which is due to rotation of dipolar group at peak temperature. Initial rise method is adopted to calculate the activation energy. Similarly the activation energy is evaluated from - log σ vs 1/T plot of electret samples. The structural investigations are made using the FTIR spectra. The spectra of virgin and electret samples are obtained in 400-4000 cm- 1 wave number ranges. Significant change in the structure is observed due to injection charge carriers. The deformation of structure is also observed. </span